Posted in Hematopathology

Periodic acid schiff (PAS) staining patterns in hematopoietic cells

Different hematopoetic cells stain differently with PAS stain. Let’s look at various PAS staining patterns in hematopoietic cells.

Pattern of PAS staining- Hematopoietic cells- CONTENTS
  1. Myeloid precursors and neutrophils
  2. Monocytes and it’s precursors
  3. Erythroid precursors and red blood cells
  4. Megakaryocytes and Platelets
  5. Lymphocytes and lymphoblasts
  6. Eosinophils and Basophils

VIEW THE SLIDE SHOW

1. MYELOID PRECURSORS AND NEUTROPHILS
Myeloid precursors show diffuse PAS positivity
Neutrophils show intense granular positivity

2.MONOCYTES AND THEIR PRECURSORS
Monocytes and their precursors show variable diffuse positivity.

3.ERYTHROID PRECURSORS AND RED BLOOD CELLS (RBCs)
Red blood cells are PAS negative
Erythroblasts show globular /granular PAS positivity
Normoblasts show diffuse PAS positivity

4. MEGAKARYOCYTES AND PLATELETS
Megakaryocytes and platelets are diffuselt PAS positive.

5. LYMPHOCYTES AND LYMPHOBLASTS
Lymphocytes show granular PAS positivity
Lymphoblasts show Block positivity

6.BASOPHILS AND EOSINOPHILS
Both basophils and eosinophils are negative for PAS

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