Welcome to your Hematoxylin and Eosin staining- QUIZ

1. Which of the following is true regarding hematoxylin and eosin staining?

A) Hematoxylin is a basic dye it binds to the nuleic acids in the nucleus of cell, imparting a beep blue to black color.

B) Eosin is a basic dye it binds to the nuleic acids in the nucleus of cell, imparting a beep blue to black color.

C) Both are true

D) None of the above

2. Which of the following is added as a preservative in Eosin solutions to prevent fungal organism growth

A) Ethanol

B) A crystal of thymol

C) Methanol

D) Picric acid

False regarding preparation and extraction of hematoxylin stain?

A) It is extracted from the heartwood of the tree Hematoxylin campechianum

B) It is extracted with hot water and precipitated out using urea

C) Hematoxylin is a stain by itself

D) None of the above is wrong

4. Which of the following hematoxylins are obtained by natural oxidation?

A) Ehrlich’s hematoxylin

B) Delafield’s hematoxylin

C) Both A and B

D) None of the above

5. What is the mordant used in meyer’s hematoxylin?

A) Mercuric Oxide

B) Iron

C) Aluminium

D) Sodium Iodate

6. Which of the following is used as a mordant in Harris hematoxylin?

A) Mercuric Oxide

B) Iron

C) Aluminium

D) Sodium Iodate

7. Which of the following mordant is most frequently used in routine histopathology staining with H and E?

A) Mercuric Oxide

B) Iron

C) Aluminium

D) Sodium Iodate

8. Which of the following hemtoxylin is routinely used for Papanicolau staining (PAP)?

A) Erhlich hematoxylin

B) Delafield hematoxylin

C) Meyer hematoxylin

D) Harris hematoxylin

9. Which of the following hematoxylins is routinely used for Frozen section?

A) Meyer hematoxylin

B) Harris hematoxylin

C) Cole hematoxylin

D) Carrazi’s hematoxylin.

10. Which of the following hematoxylin takes longest time to stain?

A) Erhlich hematoxylin

B) Delafield hematoxylin

C) Meyer hematoxylin

D) Harris hematoxylin

11. What does it mean to regressively stain a slide?

A) Staining is done to a desired intensity and no more

B) A slide is deliberately over stained and de-stained until the end point is reached.

C) Both are true

D) None of the above

A trainee stains a section swith Van-Gieson, and asks for your opinion. You observe that the nuclear staining is not clear. On enquiry the trainee says that he used Ehrlich's hematoxylin (alum hematoxylin) as counterstain for 20-25 mins. What will you advice to correct the nuclear staining?

A) Increase the duration of staining with hematoxylin

B) Use weigert’s hematoxylin or celestine blue hematoxylin sequence instead of erhlich’s hematoxylin.

C) Use a different lot of hematoxylin

D) Rest the solution for ripening

13. All of the following are examples of Iron hematoxylin except?

A) Weigert’s hematoxylin

B) Heidenhain’s hematoxylin

C) Verhöeff’s hematoxylin for elastin fibers.

D) Ehrlich hematoxylin

14. Which of the following hematoxylins is ideal in staining Myelin?

A) Weigert’s

B) Heidenhain’s

C) Loyez

D) Verhöeff’s for elastin fibers.

15. Mallory’s PTAH contains which of the following mordants?

A) Mercury

B) Tungsten

C) Iron

D) Aluminium

16. Which of the following is routinely used for differentiation of hematoxylin?

A) 1% acid alcohol

B) 100% ethanol

C) Scott tap water

D) Methanol

17. Which of the following is routinely used for blueing?

A) 1% acid alcohol

B) 100% ethanol

C) Scott tap water

D) Methanol

18. Verhoff’s hematoxylin is a type of iron hematoxylin used to stain?

A) Nucleus

B) Elastic fibres

C) Myelin

D) Mucins

19. Which of the following is the correct sequence of Hematoxylin staining?

A) Dewax parrafin sections- Rehydrate with increasing grades of alcohol—Stain with hematoxylin—Differntiate, wash and blue in alkaline tapwater– Stain with eosin—Wash, dehydrate and apply coverslip.

B) Dewax parrafin sections- Rehydrate with decreasing grades of alcohol—Stain with hematoxylin—Differntiate, wash and blue in alkaline tapwater– Stain with eosin—Wash, dehydrate and apply coverslip.

C) Stain with hematoxylin—Differntiate, wash and blue in alkaline tapwater– Stain with eosin—Wash, dehydrate and apply coverslip– Dewax parrafin sections- Rehydrate with decreasing grades of alcohol.

D) Stain with eosin—Wash, dehydrate and apply coverslip– Dewax parrafin sections- Rehydrate with decreasing grades of alcohol– Stain with hematoxylin—Differntiate, wash and blue in alkaline tapwater.

20. Which of the following factors affect hematoxylin staining?

A) Age of the staining solution

B) Duration of ripening

C) Type of mordant used

D) All of the above

Time’s up

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4 responses to “Hematoxylin and Eosin staining- QUIZ”

Ponsiano Tonya

20 Jul 2021

Please countercheck question number five, six and seven.
The question asks about mordants but the response are all about oxidants

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1. pathologymcqs
  
  21 Jul 2021
  
  The mordants for each hematoxylin type are mentioned in the answers. I can send you the reference text if you need. Kindly leave your email id. Thanks.
  
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Anonymous

31 Dec 2022

Need more mcqs.

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1. Pathology MCQs
  
  31 Dec 2022
  
  Will definitely bring more in the future!
  
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Hematoxylin and Eosin staining- QUIZ

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