Molecular techniques- quiz Posted on 9 Jul 2021 by pathologymcqs Welcome to Molecular techniques- quiz Each question carries 5 MARKS. ATTEMPT AL THE QUESTIONS GOOD LUCK !! 1. Which of the following components of a DNA take part in the coding process?A) IntronsB) TransposonsC) ExonsD) miRNA 2. What is the primary function of miRNA?A) It acts as an inhibitor of mRNAB) It activates mRNAC) It activates protein synthesisD) None 3. Which of the following anticoagulants is unsuitable for adding in the peripheral blood for DNA and RNA studies?A) EDTAB) ACDC) HeparinD) None 4. For Southern blot analysis what is the required amount of DNA required per enzymatic digestion to detect single copy of genomic targets.A) 10 microgramsB) 10 nanogramsC) 10 milligramsD) 10 picograms 5. For PCR based assay what is the required amount of DNA required per enzymatic digestion to detect single copy of genomic targets.A) 100 microgramsB) 100 nanogramsC) 100 milligramsD) 100 picograms 6. Which of the following options correctly show the sequence of events in southern blot technique?A) DNA digestion by restriction endonuclease--- Separation of fragments by electrophoresis --- Transfer DNA from cell to membrane -- Probe hybridization -- Expose to film and add substrate-- Visualization of bands.B) Separation of fragments by electrophoresis --- Transfer DNA from cell to membrane -- Probe hybridization -- Expose to film and add substrate-- Visualization of bands-- DNA digestion by restriction endonuclease.C) Transfer DNA from cell to membrane -- Probe hybridization -- Expose to film and add substrate-- Visualization of bands-- DNA digestion by restriction endonuclease-- Separation of fragments by electrophoresis.D) Expose to film and add substrate-- Visualization of bands-- DNA digestion by restriction endonuclease-- Separation of fragments by electrophoresis-- Transfer DNA from cell to membrane -- Probe hybridization. 7. What does CT (Cycle threshold) value denote with respect to PCR( Polymerase chain reaction)?A) High CT correlates with high viral loadB) Low CT value correlates with high viral loadC) BothD) None 8. What are the main indications of southern blot analysis?A) Point mutationsB) Gene deletionsC) Gene amplificationsD) All of the above 9. Which of the following is a major difference between southern and northern blotting?A) No restriction enzyme (endonuclease) is used for digestion in northern blotting.B) Electrophoresis is not done on northern blotting.C) Formaldehyde is used in Southern blottingD) None of the above 10. Which of the following is true regarding FISH( Fluorescent In-situ Hybridization)A. Minimum of 500 cells should be analysed in interphase FISHB. Minimum of 50 cells with all 46 chromosomes should be analysed in metaphase FISH.C. Ageing of fixed tissue on a glass slide is an important step in FISHD. FISH signals once obtained does not fade. Time is Up! Time's up Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading... Related Author: pathologymcqs Daily MCQ's and updates will be posted for various entrance exams View All Posts
