Pathology MCQs for various entrance exams

Welcome to Molecular techniques- quiz
Each question carries 5 MARKS.
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1. Which of the following components of a DNA take part in the coding process?

A) Introns

B) Transposons

C) Exons

D) miRNA

2. What is the primary function of miRNA?

A) It acts as an inhibitor of mRNA

B) It activates mRNA

C) It activates protein synthesis

D) None

3. Which of the following anticoagulants is unsuitable for adding in the peripheral blood for DNA and RNA studies?

A) EDTA

B) ACD

C) Heparin

D) None

4. For Southern blot analysis what is the required amount of DNA required per enzymatic digestion to detect single copy of genomic targets.

A) 10 micrograms

B) 10 nanograms

C) 10 milligrams

D) 10 picograms

5. For PCR based assay what is the required amount of DNA required per enzymatic digestion to detect single copy of genomic targets.

A) 100 micrograms

B) 100 nanograms

C) 100 milligrams

D) 100 picograms

6. Which of the following options correctly show the sequence of events in southern blot technique?

A) DNA digestion by restriction endonuclease— Separation of fragments by electrophoresis — Transfer DNA from cell to membrane — Probe hybridization — Expose to film and add substrate– Visualization of bands.

B) Separation of fragments by electrophoresis — Transfer DNA from cell to membrane — Probe hybridization — Expose to film and add substrate– Visualization of bands– DNA digestion by restriction endonuclease.

C) Transfer DNA from cell to membrane — Probe hybridization — Expose to film and add substrate– Visualization of bands– DNA digestion by restriction endonuclease– Separation of fragments by electrophoresis.

D) Expose to film and add substrate– Visualization of bands– DNA digestion by restriction endonuclease– Separation of fragments by electrophoresis– Transfer DNA from cell to membrane — Probe hybridization.

7. What does CT (Cycle threshold) value denote with respect to PCR( Polymerase chain reaction)?

A) High CT correlates with high viral load

B) Low CT value correlates with high viral load

C) Both

D) None

8. What are the main indications of southern blot analysis?

A) Point mutations

B) Gene deletions

C) Gene amplifications

D) All of the above

9. Which of the following is a major difference between southern and northern blotting?

A) No restriction enzyme (endonuclease) is used for digestion in northern blotting.

B) Electrophoresis is not done on northern blotting.

C) Formaldehyde is used in Southern blotting

D) None of the above

10. Which of the following is true regarding FISH( Fluorescent In-situ Hybridization)

A. Minimum of 500 cells should be analysed in interphase FISH

B. Minimum of 50 cells with all 46 chromosomes should be analysed in metaphase FISH.

C. Ageing of fixed tissue on a glass slide is an important step in FISH

D. FISH signals once obtained does not fade.

Molecular techniques- quiz

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