Periodic acid schiff (PAS) staining patterns in hematopoietic cells Different hematopoetic cells stain differently with PAS stain. Let’s look at various PAS staining patterns in hematopoietic cells. Pattern of PAS staining- Hematopoietic cells- CONTENTS Myeloid precursors and neutrophilsMonocytes and it’s precursorsErythroid precursors and red blood cellsMegakaryocytes and PlateletsLymphocytes and lymphoblastsEosinophils and Basophils VIEW THE SLIDE SHOW 1. MYELOID PRECURSORS AND NEUTROPHILSMyeloid precursors show diffuse PAS positivityNeutrophils show intense granular positivity 2.MONOCYTES AND THEIR PRECURSORSMonocytes and their precursors show variable diffuse positivity. 3.ERYTHROID PRECURSORS AND RED BLOOD CELLS (RBCs)Red blood cells are PAS negativeErythroblasts show globular /granular PAS positivityNormoblasts show diffuse PAS positivity 4. MEGAKARYOCYTES AND PLATELETSMegakaryocytes and platelets are diffuselt PAS positive. 5. LYMPHOCYTES AND LYMPHOBLASTSLymphocytes show granular PAS positivityLymphoblasts show Block positivity 6.BASOPHILS AND EOSINOPHILSBoth basophils and eosinophils are negative for PAS CLICK HERE FOR REVIEW QUESTIONS PARIS SYSTEM FOR REPORTING URINARY CYTOLOGY MCQs by Pathology MCQs 22 Mar 2022 Breast Pathology MCQ 1 by Pathology MCQs 12 Mar 2022 CNS Pathology case based MCQs-2 by Pathology MCQs 23 Feb 2022 Liked the page? Why not leave a tip for it’s creators Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading...
6 KEY POINTS TO REMEMBER IN ACUTE LYMPHOCYTIC LEUKEMIA (ALL) – t(5;14) 6 KEY POINTS TO REMEMBER FOR BOARDS – ACUTE LYMPHOCYTIC LEKUKEMIA- t(5;14) Basts harbour a translocation between IL3 and an IGH gene, resulting in variable eosinophiliaThis diagnosis can be made on the basis of immunophenotypic and genetic findings even if the bone marrow blast count is low.patients may present with an asymptomatic eosinophilia,Blasts may be deceptively absent in the peripheral blood.Eosinophils are a reactive popula tion and not part of the leukaemic clone.The prognosis is not considered to be different from that of other types of ALL Click below for a summary Answer this review question on ALL with the t(5;14) Review question- ALL – RGA CNS Pathology case based MCQs-1 by Pathology MCQs 22 Feb 2022 Central nervous system pathology MCQ 1 by Pathology MCQs 13 Feb 2022 Characteristics of Epithelial-Mesenchymal transition by Pathology MCQs 7 Dec 2021 For more hematology blogs- Click here Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading...
5 Differences between hyper granular (M3) and microgranular (M3v) variants of acute promyelocytic leukemia ( APML) Differences between hypergranular (M3) and microgranular (M3v) variants of acute promyelocytic leukemia ( APML) Acute Promyelocytic leukemia is an unique syndrome different from other forms of acute myeloid leukemia (AML). From clinical symptoms such as disseminated intravascular coagulation (DIC) to unique chromosomal translocation – t(15;17) the list is endless. Acute promyelocytic leukemia has two common variants- hypergranular (M3) and microgranular (M3v). It is essential to differentate the two variants because of prognostic purposes. Let’s see a few key 🔑 differences between them. 1.White blood cell counts M3v, that is the microgranular variant has a higher white blood cell count compared to hypergranular variant (M3). 2. Shape of the nucleus M3v or the microgranular variety has a bilobed/butterfly shaped nucleus. The hypergranular variant has round to oval nucleus. 3. Auer rods Auer rods may be present in M3v, but is very less compared to those present in the hypergranular variant. As the name suggests, hypergranular variant has large densely packed granules. 4. Immunophenotyping HLA-DR and CD34 are often positive in the microgranular variant in contrast to the hypergranular variant. 5. Associated mutations M3v- microgranular variant is associated with FLT3-ITD mutations whereas, the hypergranular variant (M3) is not associated with FLT3-ITD mutations. Why should you differentiate? Presence of high white blood cell counts and FLT3-ITD mutations garner a poor prognosis, hence it is necessary to distinguish between hypergranular variant of APML and microgranular variant of APML. Classic sliding morphology of microgranular APML blasts with FLT3-ITD mutation. Courtesy: Society of hemepath page: FACEBOOK Click to solve review question on the topic Watch the video for quick summary- Differences in hypergranular and microgranular variant of APML CLICK HERE FOR HEMATOLOGY BLOGS CLICK HERE FOR HEMATOLOGY QUIZZES RB-1 DELETED SOFT TISSUE TUMORS by Pathology MCQs 6 Dec 2021 SALIVARY GLAND PATHOLOGY QUIZ by Pathology MCQs 16 Nov 2021 THYROID PATHOLOGY QUIZ by Pathology MCQs 26 Oct 2021 Liked the page? Why not leave a tip for it’s creators Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading...
10 Key points to remember in NPM-1 mutated AML (Acute myeloid leukemia) Acute myeloid leukaemia (AML) with mutated NPM1 carries mutations that usually involve exon 12 of NPM1. Here are some key points to remember. Cup shaped blasts in NPM-1 mutated AML 1. There is a female predominance in this type of AML. 2. There is a strong association between both acute myelomonocytic and acute monocytic leukaemia and NPM1 mutation. 3. Charactistic cup-shaped blasts are seen. 4. Immunophenotype: AML with mutated NPM1 is character ized by high CD33 expression and variable (often low) CD13 expression. 5. Immunophenotype: KIT (CD117), CD123, and CD110 expres sion are common. Immunophenotyping of AML with NPM-1 mutations 6. Immunophenotype: HLA―DR and CD 34 is often negative. 7. Two major subgroups of AML with mutated NPM1 have been described: one with an immature myeloid immunophenotypic profile and one with a monocytic (CD36+, CD64+, CD14+) immunophenotypic profile. 8. Immunohistochemical detection of cytoplasmic NPM1 is predictive of NPM1 mutations. Remember that normally NPM-1 is expressed as nucleolar positivity. A diagnostic algorithm to diagnose AML with NPM-1 mutations. Cytogenetic testing is performed when cytoplasmic staining is observed (30-35%) 9. Cases with a normal karyotype, in the absence of FLT3―ITD mutation, have a characteristically favourable prognosis. 10. Co-occurrence of NPM1, FLT3-ITD, and DNMT3A muta tions has been associated with a particularly poor outcome. Hope you remember these key points for your boards! click here for review question Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading...