Posted in Staining

DIFFERENCE BETWEEN MORDANT AND ACCENUATOR IN STAINING

MORDANT and ACCENUATOR both are terms used in staining, for substances which enhance staining. However, both have different mechanisms of action. We will be looking at the difference between mordant and accenuator in staining.

Differences between mordant and accenuator

MORDANT

  1. MORDANT serves as a link or bridge between the tissue and the dye, to make the staining reaction possible.
  2. The mordant combines with a dye to form a colored “lake”, which in turn combines with the tissue to form a “tissuemordant-dye-complex”.
  3. Examples of mordants are potassium alum with hematoxylin in Ehrlich’s hematoxylin, and iron in Weigert’s hematoxylin.

More in staining

Click below for a quick summary

ACCENUATOR

  1. ACCENTUATOR, is not essential to the chemical union of the tissue and the dye.
  2. It does not participate in the staining reaction,
    but merely accelerates the reaction.
  3. Examples are potassium hydroxide in Loeffler’s methylene blue and phenol in carbol thionine and carbol fuchsin.
Posted in General pathology, Robbins questions

GENERAL PATHOLOGY MCQ- IRREVERSIBLE INJURY-NECROSIS updated answers.

QUESTION:

Which of the following statements is false regarding necrosis?

A) Necrosis is always pathological

B) Caseous necrosis is most common in the brain

C) Caseous necrosis is characteristic of a tubercular granuloma

D) Morphologically, necrotic cells show increased eosinophilia in a hematoxylin and eosin staining

Answer: B- Liquefactive necrosis occurs in the brain. Caseous necrosis occurs in all organs except the brain

Check this video out for detailed explanation:

Check out this awesome blog for clearing concepts:  https://theartofmed.wordpress.com/2015/05/29/pathologic-cell-injury-and-cell-death-ii-necrosis/amp/

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