Total Mesorectal Excision (TME) – Grossing essentials for Pathologists

The mesorectum is the fatty tissue surrounding the rectum, enclosed within the visceral mesorectal fascia. It contains:

  • Blood vessels
  • Lymphatics
  • Lymph nodes
  • Connective tissue

It is the primary route of local spread for rectal cancer. Hence, complete removal of the mesorectum during Total Mesorectal Excision (TME) is critical to reduce recurrence and improve survival.

Diagram illustrating mesorectum anatomy, tumor location, 5-cm margin, and total mesorectal excision in relation to the rectum.

Clinical Significance

  • Gold standard surgery for rectal cancer
  • Sharp dissection outside the mesorectal fascia (Waldeyer’s fascia)
  • Removes rectum + mesorectum + regional lymph nodes
  • Improves survival: 5-year survival ↑ from 48% to 68%
  • Reduces recurrence:
    • From 20–30% → 8–10%
    • With adjuvant therapy → as low as 2.6%
Illustration showing the anatomical lines of resection for Total Mesorectal Excision (TME), highlighting the tumor location and surrounding mesorectum.

Macroscopic Grading of Mesorectum

GradingDescription
Complete (Grade 3)- Good surgeryBulky, smooth mesorectum, no coning, no defects >5 mm, smooth CRM
Nearly complete (Grade 2)- Moderate Moderate bulk, defects >5 mm but not reaching muscularis propria, no exposed muscle
Incomplete (Grade 3)- Poor surgeryLittle bulk, defects exposing muscularis propria, irregular CRM
Anatomical comparison of complete, nearly complete, and incomplete mesorectum specimens, showing varying bulk and surface conditions.

Images to Capture

  1. Fresh specimen (anterior + posterior views)
  2. All slices laid sequentially (proximal → distal)
  3. Close-ups of:
    • Tumor near radial margin
    • CRM defects
    • Lymph node involvement

Grossing Template – TME Specimen

Specimen Handling

  • Specimen received fresh & unopened
  • Photograph specimen (anterior/posterior)
  • Open anteriorly, just above and below tumor
  • Insert formalin-soaked gauze in ends
  • Fix 72–96 hrs in formalin

Measurements & Description

ParameterDescription
Length of rectum______ cm
Mesorectal envelopeDescribe bulk, defects, smoothness
Coning*Present / Absent
Tumor size______ cm (L × W × Depth)
Distance to marginsProximal: ___ cm, Distal: ___ cm
Tumor locationAbove/below/anterior/posterior reflection
Radial margin (CRM)Closest point, smooth/moderate/very irregular

*Coning refers to the tapering of the mesorectum toward the distal end of the specimen. It indicates loss of mesorectal bulk and may reflect poor surgical technique, increasing the risk of positive margins and recurrence.


Sectioning Protocol

  • Transverse slices every 3–5 mm
  • Maintain anatomic orientation
  • Describe:
    • Tumor extent
    • Depth of invasion
    • Relationship to serosa, radial margin
    • Presence of nodes, distance to margin
    • Additional findings (polyps, diverticula)

Tissue Submission

Tissue/RegionSectioning Guideline
TumorEntire (≤5 blocks) or 1/cm
Deepest invasion≥2 blocks
Radial margin (CRM)≥2 blocks
Peritoneal relation≥2 blocks
Treated tumor site≥5 blocks including scar (post-neoadjuvant)
Proximal/Distal marginsEn face
All lymph nodesIn entirety; note bisected/trisected
Apical nodeSubmit separately if identified
Positive nodes near CRMNote distance and clock position

Pathologic Prognosticators


FeatureImportance
CRM positivity <1 mm↑ Local recurrence, ↓ survival
EMVI / PNIAssociated with metastasis
Tumor BuddingIndicates aggressive behavior
Lymph node yield ≥12TNM staging quality benchmark
Regression score (TRG)TRG 0 = Best prognosis
MLNR (Metastatic Lymph Node Ratio)Emerging alternative staging metric
Acellular mucinNot considered viable tumor

References


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One response to “Total Mesorectal Excision (TME) – Grossing essentials for Pathologists”

  1. drtarunroy580 Avatar
    drtarunroy580

    A video of grossing and taking blocks in a case of rectal malignancy

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