Types of DNA Dye

The DNA dye may be primarily of THREE types

1. The dye that binds in the minor groove of DNA
2. The dye that is intercalated within the base pair
3. Bis-intercalator

Important features often the three types of DNA dyes are summarised in the image below.

Basic Requirements of a Good DNA Dye
• Dye should be specific to DNA.
• Dye should bind stoichiometric to the DNA content.
• Dye should show strong enhancement of fluorescence after binding with
the target.

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