MICROSCOPY

Welcome to your MICROSCOPY-quiz

What does a bright field microscope mean?

A) The microscope is lit from above and viewed from below

B) The microscope is lit from below and viewed from above

C) The microscope uses natural day light for illumination

D) None of the above.

Which of the following is true regarding image on bright field microscopy?

A) Contrast is the result of sample scattering the light.

B) Typical appearance is bright sample, on a dark background

C) Contrast comes from absorption of light by the sample.

D) The specimen is lit from above and viewed from below

Advantages of using mono-ocular eyepiece microscopes are all except?

A) It is light weight

B) They are inexpensive

C) LCD camera can be attached with ease.

D) None of the above

What is the magnifying power of an eyepiece?

A) 10X

B) 20X

C) 40X

D) 25X

What is the magnifying power of an objective lens?

A) 20X

B) 40X

C) 50X

D) 30X

Which of the following objective lenses are most commonly used?

A) Achromatic

B) Apochromatic

C) Plane apochromatic

D) All of the above

Which of the following types of microscopes does not require staining to view the slide?

A) Electron microscope

B) Light microscope

C) Dark field microscope

D) Phase contrast microscope

Which of the following stains are commonly used for electron microscopy?

A) Hematoxylin and eosin

B) Uranyl acetate and lead citrate

C) Phosphotungistic acid

D) Osmium teroxide

Ideal thickness of a section used for electron microscopy is?

A) 75 nm

B) 60 nm

C) 100 nm

D) 80 nm

Which of the following is an ideal fixative used for electron microscopy?

A) 10% Formalin

B) 5% Glutaraldehyde

C) 2.5% Glutaraldehyde

D) 2% Picric acid

Which of the following fixatives are commonly used for secondary fixation in electron microscopy?

A) 10% Formalin

B) 5% Gluteraldehyde

C) 2.5% Gluteraldehyde

D) 1% Osmium tetroxide.

Polarizing microscopes work based on which of these phenomena?

A) Reflection and rarefaction

B) Rarefaction

C) Birefringence and dichroism

D) Absorption

Which of the following is used as a light source for Fluorescent microscopy?

A) Halogen lamps

B) Natural sunlight

C) Mercury vapor lamps with excitation filter lasers.

D) None of the above

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