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FIXATION AND TISSUE PROCESSING

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Pathology MCQs

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Welcome to your FIXATION AND TISSUE PROCESSING-quiz

Which are all the factors influencing tissue processing?

A) Viscocity

B) Agitation

C) Heat

d) All of the above

Which of the following represents correct stages of tissue processing?

A) Fixation—Dehydration – Clearing– Infiltration – Embedding.

B) Dehydration – Clearing– Infiltration – Embedding– Fixation

C) Clearing– Infiltration – Embedding– Fixation– Dehydration

D) Infiltration – Embedding– Fixation– Dehydration– Clearing

3. Which color does picric acid containing fixatives such as bouin’s fixative impart on the tissue?

A) Green

B) Yellow

C) Red

D) Magenta

What are qualities of a good clearing agent?

A) Will dissolve lipids

B) Can impede the wax penetration

C) Both A and B

D) None

Which of the following substances are currently used widely as clearing agent?

A) Xylene

B) Chloroform

C) Paraffin wax

D) Alcohol

Which of the following is routinely used as an embedding agent for electron microscopy?

A) Agar

B) Paraffin

C) Resin

D) Celloidin

Which of the following are important maintenance tips in microwave processor maintenance?

A) Any spillage or overflow should be cleaned immediately.

B) Accumulation of wax on any surface should be removed.

C) Paraffin wax bath temperatures should be monitored daily.

E) All of the above.

Which of the following is the ideal section thickness for preparing routine H and E stained sections?

A) 3-5 microns

B) 8-12 microns

C) 2 microns

D) 0.35 microns

Which of the following is the ideal section thickness for preparing sections for tissues to be examined for amyloid deposits?

A) 3-5 microns

B) 8-12 microns

C) 2 microns

D) 0.35 microns

Which of the following is the ideal section thickness for kidney biopsies?

A) 3-5 microns

B) 8-12 microns

C) 2 microns

D) 0.35 microns

Which of the following additives are applied in a glass slide as a bonding agent, to make the tissue adhere well to the glass slide?

A) Xylene

B) Poly-L-lysine

C) Alcohol

D) Formalin

Which of the following is true regarding Base- sledge microtome?

A) The specimen is held stationary and the knife slides across the specimen

B) Is able to procure thin sections 1-2 microns

C) The knife is stationary and the specimen slides across

D) Used primarily for small tissue blocks.

Which of the following microtomes are commonly used in cryostats.

A) Rotatory microtome

B) Base sledge microtome

C) Rotary rocking microtome

D) Sliding microtome

Which of the following microtomes are exclusively used in electron microscopy?

A) Rotatory microtome

B) Base sledge microtome

C) Rotary rocking microtome

D) Ultra-microtome

What is the melting point of wax?

A) 10-30 degree celsius

B) 20-30 degree celsius

C) 40-70 degree celsius

D) 0-20 degree celsius

What is the temperature of the floatation bath?

A) 10-30 degree celsius

B) 30-45 degree celsius

C) 40-70 degree celsius

D) 0-20 degree celsius

Which of the following substances/techniques is used to freeze the tissue (snap freezing)?

A) Liquefied nitrogen (−190°C).

B) Isopentane (2-methylbutane) cooled by liquid nitrogen (−150°C).

C) Dry ice (−70°C).

D) All of the above

Which of the following is true regarding Sliding microtome?

A) The specimen is held stationary and the knife slides across the specimen

B) Is able to procure thin sections 1-2 microns

C) The knife is stationary and the specimen slides across

D) Used primarily for small tissue blocks.

Cryostat sections are cut at an approximate thickness of?

A) 3-5 microns

B) 8-12 microns

C) 2 microns

D) 0.35 microns

Time’s up

Time is Up!

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8 responses to “FIXATION AND TISSUE PROCESSING”

Dr. Tarun Roy

31 Dec 2022

I wish to get more varieties of questions.

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1. Pathology MCQs
  
  31 Dec 2022
  
  Noted!
  
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deng ruot

26 Jan 2023

i am very interested to gain more tissue processing

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1. Pathology MCQs
  
  29 Jan 2023
  
  We shall add some more in the future. Thanks for the suggestion
  
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  Reply
Anonymous

29 Jan 2023

to get more mcqs regarding the fixatives and tissue processing

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Reply
1. Pathology MCQs
  
  29 Jan 2023
  
  We shall add some more in the future. Thanks for the suggestion
  
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  Reply
2. Anonymous
  
  28 Nov 2023
  
  Types of fixation tissue
  
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  Reply
Sampath KS

7 Apr 2024

Excellent Questions!

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