FIXATION AND TISSUE PROCESSING Welcome to your FIXATION AND TISSUE PROCESSING-quiz Which are all the factors influencing tissue processing? A) Viscocity B) Agitation C) Heat d) All of the above Which of the following represents correct stages of tissue processing? A) Fixation—Dehydration – Clearing-- Infiltration – Embedding. B) Dehydration – Clearing-- Infiltration – Embedding-- Fixation C) Clearing-- Infiltration – Embedding-- Fixation-- Dehydration D) Infiltration – Embedding-- Fixation-- Dehydration-- Clearing 3. Which color does picric acid containing fixatives such as bouin’s fixative impart on the tissue? A) Green B) Yellow C) Red D) Magenta What are qualities of a good clearing agent? A) Will dissolve lipids B) Can impede the wax penetration C) Both A and B D) None Which of the following substances are currently used widely as clearing agent? A) Xylene B) Chloroform C) Paraffin wax D) Alcohol Which of the following is routinely used as an embedding agent for electron microscopy? A) Agar B) Paraffin C) Resin D) Celloidin Which of the following are important maintenance tips in microwave processor maintenance? A) Any spillage or overflow should be cleaned immediately. B) Accumulation of wax on any surface should be removed. C) Paraffin wax bath temperatures should be monitored daily. E) All of the above. Which of the following is the ideal section thickness for preparing routine H and E stained sections? A) 3-5 microns B) 8-12 microns C) 2 microns D) 0.35 microns Which of the following is the ideal section thickness for preparing sections for tissues to be examined for amyloid deposits? A) 3-5 microns B) 8-12 microns C) 2 microns D) 0.35 microns Which of the following is the ideal section thickness for kidney biopsies? A) 3-5 microns B) 8-12 microns C) 2 microns D) 0.35 microns Which of the following additives are applied in a glass slide as a bonding agent, to make the tissue adhere well to the glass slide? A) Xylene B) Poly-L-lysine C) Alcohol D) Formalin Which of the following is true regarding Base- sledge microtome? A) The specimen is held stationary and the knife slides across the specimen B) Is able to procure thin sections 1-2 microns C) The knife is stationary and the specimen slides across D) Used primarily for small tissue blocks. Which of the following microtomes are commonly used in cryostats. A) Rotatory microtome B) Base sledge microtome C) Rotary rocking microtome D) Sliding microtome Which of the following microtomes are exclusively used in electron microscopy? A) Rotatory microtome B) Base sledge microtome C) Rotary rocking microtome D) Ultra-microtome What is the melting point of wax? A) 10-30 degree celsius B) 20-30 degree celsius C) 40-70 degree celsius D) 0-20 degree celsius What is the temperature of the floatation bath? A) 10-30 degree celsius B) 30-45 degree celsius C) 40-70 degree celsius D) 0-20 degree celsius Which of the following substances/techniques is used to freeze the tissue (snap freezing)? A) Liquefied nitrogen (−190°C). B) Isopentane (2-methylbutane) cooled by liquid nitrogen (−150°C). C) Dry ice (−70°C). D) All of the above Which of the following is true regarding Sliding microtome? A) The specimen is held stationary and the knife slides across the specimen B) Is able to procure thin sections 1-2 microns C) The knife is stationary and the specimen slides across D) Used primarily for small tissue blocks. Cryostat sections are cut at an approximate thickness of? A) 3-5 microns B) 8-12 microns C) 2 microns D) 0.35 microns Time is Up! Time's up Share this:FacebookTelegramWhatsAppMoreLinkedInTwitterLike this:Like Loading...