GROSSING- mcq

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Why is decalcification necessary in histopathology

A) Decalcification enables the histotechnologist to cut soft sections of the bone using the microtome, so that they can be processed like any other soft tissue of the body

B) Decalcification interferes with staining

C) It is usually seen in soft tissues

D) None of the above

None

Which of the following solutions is a better alternative to formalin in fixing bone marrow biopsies?

A) Bouin’s solution

B) Picric acid

C) Osmium tertoxide

D) Chloroform.

None

Types of decalcifying agents are?

A) strong mineral acids

B) weaker organic acids

C) chelating agents.

D) All of the above

None

Which of the following is a rapid decalcifying agent?

A) EDTA

B) Formic acid

C) HCl

D) Nitric acid

None

Which of the following is the preferred decalcifying agent for IHC and electron microscopy?

A) EDTA

B) Formic acid

C) HCl

D) Nitric acid

None

How does temperatures affect decalcification?

A) Increased temperatures decrease decalcification

B) Increase in temperatures increase decalcification

C) Temperature does not affect decalcification

D None

None

In case a specimen is placed in the incorrect casette during grossing which factors can help identify the correct specimen?

A) Type of tissue mentioned in the gross form

B) Measurements mentioned in the grossing form

C) Number of fragments mentioned in the grossing form.

D) All of the above

None

If all the parameters mentioned in the above question are similar then, what can be done to identify tissue mixing?

A) Immunoperoxidase studies for ABH blood group antigens

B) HLA typing using PCR

C) Polymorphic microsatellite markers

D) All of the above

None

What is a floater?

A) Extraneous tissue consists of fragments of tissue that are present on a slide but are derived from a different specimen.

B) Unfixed specimen

C) Improperly grossed specimen

D) None of the above

None

Which of the following are methods used to prevent floaters?

A) All dissecting tools (forceps, scissors, scalpel) should be kept in a jar of water between uses.

B) The dissection area must be kept clean.

C) Solutions in tissue processors should be changed routinely

D) All of the above.

None

Accepted major discrepancy rate among intra operative consultations compared with standard sections permitted by college of American pathologists( CAP) is?

A) 10%

B) 5%

C) 3%

D) 15%

None

Which of the following micro-organisms have been found to to transmitted to healthcare personnels by means of aerosols from the specimen ?

A) Mycobacterium tuberculosis

B) Plasmodium

C) Parvovirus-B19

D) Borrelia

None

Seroconversion is highest after needle- prick injury in which of the following infections?

A) Hepatitis A

B) Hepatitis B

C) Hepatitis C

D) HIV

None

Which of the following are methods used to measure microscopic sizes?

A) Direct measurement on the slide

B) Vernier scale on movable stage

C) Ocular reticle (graticule)

D) All of the above

None

Which of the following is the most accurate method to determine microscopic sizes?

A) Direct measurement on the slide

B) Vernier scale on movable stage

C) Ocular reticle (graticule)

D) Estimation of known field diameters

None

Which of the following is a prerequisite to denying a specimen reception?

A) Incorrect or no patient identification,

B) mismatch in the number of specimens mentioned on the form and those received

C) investigation not offered by the laboratory

D) All of the above

None

Which of the following tissues require mild Decalcifying agent along with 10% Neutral buffered formalin (NBF)

A) Intestines

B) Trachea

C) Skin

D) Lungs

None

Which of the following is true regarding specimens labelled as biohazard?

A) Personal hygiene and protective clothing should be worn

B) Wire mesh or Klevar gloves can be worn to prevent cuts

C) Specimen should not be opened immediately after receiving.

D) After a biohazardous specimen is grossed, the grossing surface is cleaned with 2% hypochlorite solution and the instruments are immersed in 10% hypochlorite solution for 30 minutes.

None

In which of the following biomedical waste disposal bags is specimen waste generated from grossing discarded?

A) RED

B) YELLOW

C) BLACK

D) NONE

None

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