SPECIAL STAINS- ASCP questions

Welcome to your SPECIAL STAINS- QUIZ

1. Which of the following statements are true regarding Supra-vital staining?

A) It is a method of staining used to examine living cells

B) Living cells do not take up supra vital stains only dead cells do.

C) Janus green is a commonly used supra vital stain

D) It is done by injecting the dye into any part of the human body.

None

2. Which of the following dyes is useful in detecting DNA and RNA?

A) Alizarin Red

B) Acridine orange

C) Alcian blue

D) Acridine red

None

3. Prussian blue staining is used to stain which of the following metals?

A) Copper

B) Zinc

C) Iron

D) Selenium

None

4. All are true about toluidine blue stain except?

A) It is a metachromatic stain

B) Used to stain the nissil granules

C) It can be used to stain mast cell granules

D) It cannot be used as a nuclear stain.

None

5. All of the following stains can be used to stain lipophilic tissues except?

A) Oil red O

B) Orcein

C) Methylene violet

D) Osmium tertroxide.

None

6. Substances containing which of the following groups are oxidized by periodic acid?

A) 1-2 glycols

B) 1-4 glycols

C) 1-6 glycols

D) 1-8 glycols

None

7. All of the following substances are PAS positive except?

A) Neutral mucins

B) Lipids

C) Tissue basement membranes

D) Fungal organisms

None

8. What are the three basic components of Schiff’s reagent or Basic fuscin?

A) Rosanilin

B) Pararosanilin

C) Magenta II

D) All of the above

None

9. Which of the following is true regarding PAS and Alcian blue staining?

A) The two stains in combination are used to differentiate neutran and acid mucins

B) Alcian blue stains neutral mucins

C) PAS stains acid mucins

D) None of the above

None

10. Feulgen staining is specific for?

A) RNA

B) DNA

C) Proteins

D) Carbohydrates

None

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